anti smad3 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
anti smad3
Anti Smad3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+smad3/Smad3+Rabbit+mAb/pmc12914543-97-110-112
Average 96 stars, based on 1111 article reviews
Anti Smad3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+smad3/Smad3+Rabbit+mAb/pmc12914543-97-110-112
Average 96 stars, based on 1111 article reviews
anti smad3 - by Bioz Stars,
2026-09
96/100 stars
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Incubation:Article Title: Methods of treatment with CD8 T cell-mediated immune therapy Article Snippet: Briefly, chromatin from fixed cells was sonicated by Vibra-cell VC130 (Sonics&Materials) for 2 cycles of 6 sec ON and 30 sec OFF at 6-9 output watt. .. Chromatin was incubated with anti-rabbit IgG (1:500), anti-Smad2 (1:50), or Article Title: Serpine1 Regulates the Enhanced Inhibitory Effect of CHIR99021 Combined with Fibroblast Growth Factor 2 on Myocardial Fibrosis After Myocardial Infarction in Mice. Article Snippet: Total proteins were separated by 10% SDS–PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Biosharp, Beijing, China). .. The membranes were blocked with 5% bovine serum albumin in Tris-buffered saline https://doi.org/10.3390/ijms27041627 and incubated overnight at 4 ◦C with the following primary antibodies: anti-Serpine1 (HA500124, HuaBio, 1:1000, China), anti-α-SMA (ET1607-53, HuaBio, 1:1000, China), antiColI (HA722517, HuaBio, 1:1000, China), anti-ColIII (HA720050, HuaBio, 1:1000, China), anti-Smad2 (ET1604-22, HuaBio, 1:5000, China), anti-p-Smad2 (ET1612-32, HuaBio, 1:5000, China), Article Title: TGIF2-mediated HMGB3 overexpression promotes esophageal squamous cell carcinoma proliferation and metastasis through TLR3/TGF-β signaling Article Snippet: Next, proteins were transferred to nitrocellulose membranes (Millipore; Temecula, California, USA). .. Afterward, before incubation with primary antibodies overnight at 4 °C, membranes were blocked with 5% non-fat milk at 37 °C for 1 h. Subsequently, membranes were treated with horseradish peroxidase-conjugated secondary antibodies against rabbit or mouse IgG (Abcam, Massachusetts, USA, 1:5000) at 37 °C for 1 h. The following primary antibodies were used to assess the expression of proteins: anti-β-actin (#3700; Cell Signaling Technology, Massachusetts, USA, 1:5000), anti-TGIF2 (#ab190152; Abcam, 1:1000), anti-p-TGIF2 and TGIF2 (#sc-390870; Santa Cruz, CA, USA), anti-HMGB3 (#ab75782; Abcam, 1:1000), anti-TLR3 (#ab62566; Abcam, 1:1000), anti-TGF-β (#ab215715; Abcam , 1:1000), anti-SMAD2/3 (#8685; Cell Signaling Technology, 1:1000), anti-SMAD2 (#5339; Cell Signaling Technology, 1:1000), anti-p-SMAD2 (#3108; Cell Signaling Technology, 1:1000), Immunoprecipitation:Article Title: Methods of treatment with CD8 T cell-mediated immune therapy Article Snippet: Briefly, chromatin from fixed cells was sonicated by Vibra-cell VC130 (Sonics&Materials) for 2 cycles of 6 sec ON and 30 sec OFF at 6-9 output watt. .. Chromatin was incubated with anti-rabbit IgG (1:500), anti-Smad2 (1:50), or Article Title: TRPA1 promotes overactive bladder progression by activating the NLRP3 inflammasome and driving pyroptosis. Article Snippet: ChIP assays were performed following the standard procedure using SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology, USA). .. Briefly, treated cells were cross-linked with 1% formaldehyde, and the prepared chromatin was then immunoprecipitated with anti-MAZ (1:50, Active Motif, China), Article Title: TRPA1 promotes overactive bladder progression by activating the NLRP3 inflammasome and driving pyroptosis. Article Snippet: After centrifugation, the supernatant was incubated with an anti-TRPA1 antibody for 2 hours before the addition of Protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology, USA) and subsequent overnight incubation at 4 °C. .. The immunoprecipitation samples were verified by western blot with anti-MAZ (1:100, Active Motif, China), Saline:Article Title: Serpine1 Regulates the Enhanced Inhibitory Effect of CHIR99021 Combined with Fibroblast Growth Factor 2 on Myocardial Fibrosis After Myocardial Infarction in Mice. Article Snippet: Total proteins were separated by 10% SDS–PAGE and transferred onto polyvinylidene difluoride (PVDF) membranes (Biosharp, Beijing, China). .. The membranes were blocked with 5% bovine serum albumin in Tris-buffered saline https://doi.org/10.3390/ijms27041627 and incubated overnight at 4 ◦C with the following primary antibodies: anti-Serpine1 (HA500124, HuaBio, 1:1000, China), anti-α-SMA (ET1607-53, HuaBio, 1:1000, China), antiColI (HA722517, HuaBio, 1:1000, China), anti-ColIII (HA720050, HuaBio, 1:1000, China), anti-Smad2 (ET1604-22, HuaBio, 1:5000, China), anti-p-Smad2 (ET1612-32, HuaBio, 1:5000, China), Expressing:Article Title: TGIF2-mediated HMGB3 overexpression promotes esophageal squamous cell carcinoma proliferation and metastasis through TLR3/TGF-β signaling Article Snippet: Next, proteins were transferred to nitrocellulose membranes (Millipore; Temecula, California, USA). .. Afterward, before incubation with primary antibodies overnight at 4 °C, membranes were blocked with 5% non-fat milk at 37 °C for 1 h. Subsequently, membranes were treated with horseradish peroxidase-conjugated secondary antibodies against rabbit or mouse IgG (Abcam, Massachusetts, USA, 1:5000) at 37 °C for 1 h. The following primary antibodies were used to assess the expression of proteins: anti-β-actin (#3700; Cell Signaling Technology, Massachusetts, USA, 1:5000), anti-TGIF2 (#ab190152; Abcam, 1:1000), anti-p-TGIF2 and TGIF2 (#sc-390870; Santa Cruz, CA, USA), anti-HMGB3 (#ab75782; Abcam, 1:1000), anti-TLR3 (#ab62566; Abcam, 1:1000), anti-TGF-β (#ab215715; Abcam , 1:1000), anti-SMAD2/3 (#8685; Cell Signaling Technology, 1:1000), anti-SMAD2 (#5339; Cell Signaling Technology, 1:1000), anti-p-SMAD2 (#3108; Cell Signaling Technology, 1:1000), Western Blot:Article Title: TRPA1 promotes overactive bladder progression by activating the NLRP3 inflammasome and driving pyroptosis. Article Snippet: After centrifugation, the supernatant was incubated with an anti-TRPA1 antibody for 2 hours before the addition of Protein A/G PLUS-Agarose beads (Santa Cruz Biotechnology, USA) and subsequent overnight incubation at 4 °C. .. The immunoprecipitation samples were verified by western blot with anti-MAZ (1:100, Active Motif, China), Article Title: MMP9 regulates osteogenesis and MMP2 expression through the TGF-β1/SMAD2/3 signaling pathway in lipopolysaccharide-induced osteoblasts Article Snippet: Anti-phospho-SMAD2 and anti-phospho-SMAD3 antibodies for immunofluorescence were purchased from Abcam (UK). .. The anti-phospho-SMAD2, anti-phospho-SMAD3, anti-SMAD2, anti-SMAD3, and anti-RUNX2 antibodies for WB were purchased from Cell Signaling Technology (USA). .. The anti-SMAD2 and anti-SMAD3 antibodies for EMSA were purchased from Cell Signaling Technology. |
